Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.

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As observed in Fig. The results have established the biorrewctor potential of ethanol production by continuous fermentation with immobilized cells in waste lignocellulosic materials. We could appreciate some differences between the surfaces of the four carriers. The characterization made it possible to establish the microscopic structural differences between the four materials, as well as the differences in composition of lignin, cellulose, hemicelluloses and ash.

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However, an industrial implementation of this process requires further optimizations that allow for one to increase the amount of cells immobilized on the carriers, as well as improvements in the operational conditions of the process. The amount of immobilized biomass was established by the modified dry-weight technique [9]. This study is focused on the development of an immobilization process of yeast cells in waste lignocellulosic materials and their evaluation in the ethanol production by using packed bed bioreactors.

Most industrial processes for ethanol production use batch systems, which are being implemented in Colombia in new production plants [4]. These results indicate that, despite of the total absence of sugars at the end of fermentation, not all of them were converted to ethanol. This Acid was analyzed and identified by Spectroscope method called Ultra Violet Visible corroborating its existence. Subsequently, the bioreactor was filled with fresh culture medium and allowed to operate with recirculation for 12 hours [19].


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Conclusions In this study we developed a protocol for quantification of immobilized biomass on lignocellulosic carriers and we obtained reproducible results.

These results are very relevant if compared with the values obtained in the traditional batch fermentations as well as the results obtained by other researches, who used different materials in the immobilization of cells for the production of ethanol [11—20].

The batch fermentation elapsed time was 12 hours and it was performed in a bioreactor in triplicate. buorreactor

Continuous fermentation For continuous fermentation, a feeding flow of 1. Figures 4 and 5 show the results obtained for the continuous fermentation carried out with sucrose.

The aim of this study empacaado to establish a process of cell immobilization on lignocellulosic waste and their potential use in the production of ethanol. Immobilization matrices Empacadi as immobilization matrices were lignocellulosic materials, sugar cane bagasse, corn leaves, corn cobs and wood shavings, obtained as agro-industrial waste.

It is observed that the cane bagasse was the material in which the highest amount of cells was immobilized, 0. We took the sample at the end of the fermentation. Martins dos Santos, E.

Journal of Agricultural of Food Chemistry. The results obtained have allowed us to establish the potential this lignocellulosic waste has as carriers for cell immobilization for the continuous production of ethanol, and the possibility of using glucose syrup as an alternative substrate. Electrochemically and Bioelectrochemically Induced Ammonium Recovery.

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Un biorreactor Novela de alta densidad de cultivo de diversas comunidades microbianas

We used glucose as a carbon source to establish the reference conditions and we made the fermentations with commercial sucrose. Please sign in or create an account.


For other languages click here. Based on these results we chose the biomass quantification protocol modified dry weight treatmentthat uses NaOH biorreactlr. Treatment column corresponds to the different solutions evaluated NaOH 0. The results of the fermentations carried out with glucose syrup obtained from cassava flour are shown in Figs.

An important result in this experimentation was the confirmation of reproducibility of operational conditions of fermentations and stability of packed bed system during operation time. Study of the effect of size and flow rate for each material To determine the effect on immobilization of flow rate and the carrier size, we performed a factorial experimental design with 2 factors and 2 levels, and a central point with three replicates.

There are also demanding requirements for materials used as carriers, since chemical, physical and biological stability has to be ensured, and the carriers and the technique must be low cost and easy to implement on an industrial empacxdo. However, to achieve better results and be able to implement this system at industrial level, it is necessary to optimize the process to establish the best concentrations of sugars, inoculum size and biocatalyst quantity appropriate for the bioreactor, improving the yields and making the process competitive with the actual existent ethanol production technology.

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